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Malaria Journal volume 13 , Article number: Cite this article. Metrics details. Intensified efforts are urgently needed to contain and eliminate artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion. As a first step, the prevalence of Plasmodium spp.

A cross-sectional population-based study using a two-stage cluster sampling was conducted in the rural districts of Chhaeb and Chey Saen, from September to October In each district, 30 clusters of 10 households were randomly selected.

In total, blood samples were collected for 1, participants in Chhaeb and 1, in Chey Saen. Prevalence of Plasmodium spp. The prevalence of Plasmodium spp. Twenty-seven samples were positive for P. Only 4. The overall prevalence of P. The prevalence might be underestimated because of the absentees — mainly forest workers - and the workers of private companies who were not included in the study. These results confirm the need to urgently develop and implement targeted interventions to contain and eliminate P.

Over the last decade, huge efforts have been made to control malaria. Several countries are now classified by the World Health Organization WHO as being in the malaria pre-elimination or elimination phase. Such declining trends have been observed in the Greater Mekong subregion[ 2 ], including Cambodia. Unfortunately, these achievements are threatened by the emergence of artemisinin-resistant parasites in the region[ 3 — 5 ], initially detected in Pailin, Cambodia, near to the Thai-Cambodian border[ 6 ].