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BMC Cancer

Loading ePaper. Table of contents Plenary lectures PL Plenary lectures PL PL Local protein synthesis in neurons Erin Schuman1 1Max Planck Institute for Brain Research, Synaptic Plasticity, Frankfurt, Germany The complex morphology of neurons, with synapses located very far from the cell body, necessitates the localization of important cell biological machines and processes within dendrites and axons. I will discuss our studies on the localization and translation of mRNAs at synapses and how local proteomes contribute to the diversity of brain synapses.

Hyperactive kinase activity is one of the drivers of tumorigenesis and cancer progression and results in altered phosphorylation abundance and activity of downstream substrates. A prime prerequisite for personalized treatment is that kinase activity inference can be based on a single patient sample. This is pivotal in a clinical setting with the aim to select the best kinase inihibitor treatment for individual patients.

To this end, phosphoproteomics coupled to INKA analysis has emerged as an advantageous approach that achieves an optimized ranking of inferred kinase activities in single samples www. INKA integrates kinase phosphorylation and substrate phosphorylation to infer kinase activity.

The output is a ranked kinase activity bar graph and an individualized kinase-substrate network that can be used by biologists and clinicians to extract actionable information from modern deep comprehensive patient phosphoproteomic data. The analyses offer explanation for lack of drug response and uncover actionable resistance mechanisms. Ultimately, prospective clinical trials that include drug selection based on patient phosphoproteomic data coupled to inference, will show the added value of phosphoproteomics combined with genomics for precision medicine.

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