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A, There is no statistic difference between renalase expression between the ACEI and control groups both in the ischemia kidney and the nonischemia one. B, Western blotting showing renalase protein expression of ischemia kidney is lower than that of the nonischemia one.

Alpha-tubulin also shown as a housekeeping control. Alpha-tubulin expression is also shown as a housekeeping control. B , Clearance rate of norepinephrine at conditions of different perfusion flow. D , Renalase activity and concentration in the perfusate at different time points during the perfusion.

The activity and concentration are undetectable at time 0. But within 10 minutes of the initiation of norepinephrine perfusion, renalase activity and concentration increased significantly and remained high after 20 minutes during the perfusion procedure. A , Plasma norepinephrine concentration in different groups 0. B , Plasma renalase concentration in different groups 0.

Here, we identify a potential mechanism underlying norepinephrine accumulation in a rat model of heart failure. Renalase expression is demonstrated immunohistochemically in kidney cortex A—C and medulla D—F.