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With a microscope weight below 3 g and a highly flexible connection cable, MINI2P allowed stable imaging with no impediment of behavior in a variety of assays compared to untethered, unimplanted animals. The improved cell yield was achieved through a optical system design featuring an enlarged field of view FOV and a microtunable lens with increased z -scanning range and speed that allows fast and stable imaging of multiple interleaved planes, as well as 3D functional imaging.

Successive imaging across multiple, adjacent FOVs enabled recordings from more than 10, neurons in the same animal. Large-scale proof-of-principle data were obtained from cell populations in visual cortex, medial entorhinal cortex, and hippocampus, revealing spatial tuning of cells in all areas. A major goal of contemporary neuroscience is to identify the neural population codes underlying complex mammalian brain functions.

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