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To date, cortisol assessment in hair samples has been reported as a validated method to evaluate hypothalamic-pituitary-adrenal HPA axis activity in humans and animals. The objectives of this study were to evaluate changes in hair cortisol concentrations relative to the beginning of semen production and the effects of hair cortisol concentrations on semen quality of bulls.

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Alejandro Villa-Godoy. Tracy Burnett. Cortisol has long been used as a marker of the stress response in animals. Cortisol can be analyzed from different media, most notably from the blood, saliva, and feces; however, the collection of cortisol from some of these media requires invasive procedures or excessive handling of the animals.

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